Anti-LPA Recombinant Antibody (LT3015 [B7(LT3000, Lpathomab)])
CAT#: TAB-0340CL
This humanized antibody LT3015 is generated from the high-affinity, specific anti-LPA mouse mAb LT3000, also called Lpathomab. The target of the antibody lysophosphatidic acid is a bioactive lipid mediating multiple cellular responses, and it's involved in the establishment and progression of cancer by providing a pro-growth tumor microenvironment and promoting angiogenesis. The EC₅₀ of the humanized antibody to 12:0 (lauroyl) LPA was determined to be 75.6 ng/mL by ELISA.
Published Data
Tested Data
Gene Expression
Figure 1 LPA binding affinity measured as in Figure for native LT3015 (WT) and three LT3015 single point mutations.
Fleming, J. K., Wojciak, J. M., Campbell, M. A., & Huxford, T. (2011). Biochemical and structural characterization of lysophosphatidic Acid binding by a humanized monoclonal antibody. Journal of molecular biology, 408(3), 462-476.
Figure 2 Site-directed mutagenesis and LPA binding assays of LT3015.
In comparison to native LT3015 (WT), the introduction of mutations in the LT3015 heavy chain (AsnH52Tyr/SerH52Tyr) or light chain (AnsL30Arg) does not significantly increase LPA affinity. However, their combination (AsnH52Tyr/SerH54Tyr/AsnL30Arg) results in a modified version of the antibody with increased LPA binding affinity.
Fleming, J. K., Wojciak, J. M., Campbell, M. A., & Huxford, T. (2011). Biochemical and structural characterization of lysophosphatidic Acid binding by a humanized monoclonal antibody. Journal of molecular biology, 408(3), 462-476.
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SDS-PAGE
Figure 1 Anti-LPA Recombinant Antibody (TAB-0340CL) in SDS-PAGE
SDS-PAGE analysis of TAB-0340CL in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Anti-LPA Recombinant Antibody (TAB-0340CL) in HPLC
The purity of this antibody is> 95% as determined by SEC-HPLC.
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Subcellular Location and Protein Expression
Figure 1 IHC staining of human liver
Immunohistochemical staining of human liver shows moderate cytoplasmic and membranous positivity in hepatocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60604/144547_A_8_4_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human kidney
Immunohistochemical staining of human kidney shows distinc extra cellular and some, weaker membranous and cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16072/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60604/144547_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60604/144547_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Leydig cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60604/144547_A_4_6.jpg
Normal Tissue
Figure 6 Skin
Epidermal cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60604/144547_B_6_8.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16072/36402_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198670-LPA
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Specifications
- Host Species
- Human
- Type
- Humanized Antibody
- Clone
- LT3015 [B7(LT3000, Lpathomab)]
- Related Disease
- Macular degeneration, pain-related disorders, malignancies, angiogenesis, fibrosis-related disorders
Target
- Alternative Names
- LPA; Lysophosphatidic acid
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(Creative Biolabs Cat# TAB-0340CL, RRID: AB_3111766)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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